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Luteinising Hormone (LH)

Paediatrics

Assessment of the hypothalamic-pituitary-gonadal axis.

General information

Collection container: Lithium heparin plasma (Sarstedt orange top, 4.9 mL adults/1.2 mL paediatrics) Serum (Sarstedt brown top 4.9 mL adults/white top 1.2 mL paediatrics).

Type and volume of sample: 1.0 mL whole blood is required, as a minimum volume, for the analysis of LH and FSH.

Specimen transport/special precautions: The tube should be thoroughly mixed before transport to the lab.

Laboratory information

Method principle: The assay is a solid-phase two-site fluoroimmunometric assay. LH in the sample reacts with an immobilised monoclonal antibody specific for the ß-subunit. Bound LH is then reacted with a Europium labelled monoclonal antibody specific for a different site on the ß-subunit. Enhancement solution dissociates Europium ions from the labelled antibody into solution where they form fluorescent chelates and the resultant level of fluorescence is directly proportional to the quantity of LH in the sample.

Biological reference ranges:

0-2 years <3.5 IU/L
2-10 years <0.3 IU/L
Post pubertal male 1.7-8.6 IU/L
Post pubertal female - follicular follicular 2.4-12.6 IU/L
mid-cycle 14-95.6 IU/L
luteal 1.0-11.4 IU/L


Concentration of serum LH and FSH (AutoDELFIA assays), expressed as mean and 5th and 95th percentiles, in normal subjects at different pubertal stages (n=316 for basal levels, n=106 for GnRH stimulated levels):

Pubertal stage Males Females
Basal GnRH-stimulated peak Basal GnRH-stimulated peak
LH (IU/L) FSH (IU/L) LH (IU/L) FSH (IU/L) LH (IU/L) FSH (IU/L) LH (IU/L) FSH (IU/L)

T11(>2.6yr)

<0.6 1.0 (1.0-1.4) N/A N/A <0.6 3.7 (1.0-8.3) N/A N/A
T12 <0.6 1.1 (1.1-1.6) 2.2 (1.1-3.3) 5.7 (2.4-10.6) <0.6 1.6 (1.0-3.4) 2.1 (0.6-4.2) 11.7 (1.9-27.1)
TII 1.3 (0.6-2.7) 1.8 (1.0-4.3) 15.6 (1.9-31.0) 3.6 (1.4-10.2) 1.0 (0.6-?) 2.3 (1.0-4.8) 5.3 (0.6-12.5) 6.5 (1.8-13.2)
TIII 1.4 (0.6-2.5) 2.1 (1.0-5.5) 16.1 (7.3-32.0) 4.2 (1.1-13.0) 2.9 (0.6-5.0) 3.9 (2.6-5.1) 21.0 (14.6-31.0) 7.9 (5.9-12.0)
TIV 1.6 (0.7-2.5) 2.1 (1.0-5.2) 17.3 (12.0-28.0) 4.8 (1.7-12.0) 3.1 (1.0-6.0) 4.0 (1.5-7.2) 26.2 (10.4-54.5) 8.6 (4.0-18.0)
TV 4.7 (2.4-8.2) 3.2 (12-5.7) 28.9 (9.5-56.3) 5.3 (1.8-12.0) 5.7 (0.6-15.4) 4.1 (1.0-7.3) 37.9 (9.7-114.0) 9.2 (2.8-18.8)

 

Turnaround time: 3 weeks

Clinical information

LH or hLH (Luteinising Hormone) is a glycoprotein, synthesised and secreted by the anterior pituitary, whose main function is to stimulate steroid production in the ovary and testis. LH production is low in childhood, increasing to adult levels during puberty. Levels in adult males are relatively constant under feedback control, but levels in adult females undergo cyclical variation, reaching very high values during the mid-cycle peak. After the menopause levels are persistently elevated. Since normal levels in childhood are low, stimulation tests using LHRH are needed to investigate hypofunction.

Factors known to significantly affect the results:

  • Samples collected into EDTA and citrate must not be used. This is due to their chelating action upon the Europium used within the assay.
  • This assay should not be used to measure hLH concentrations during pregnancy or immediately post-partum interferes due to interference from hCG.
  • Grossly haemolysed samples are unsuitable for analysis.

 

(Last reviewed 29th February 2016)