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Double stranded DNA antibodies (IgG)

General information

The presence of autoantibodies to double stranded DNA is strongly suggestive of SLE, although they are detected in only 40-60% of patients with this disease.

Specimen transport: At room temperature

Repeat frequency: We recommend not repeating this test more than once a month, unless the patient is
undergoing plasmapheresis

Special precautions: None

Laboratory information

Normal reference range: <0-0.99 iu/mL

Volume and sample type: 7ml clotted blood

Method: Multiplex flow immunoassay and Immunofluorescence

Turnaround time (calendar days from sample receipt to authorised result): Median - 1

Clinical information

Indications for the test: Diagnosis and monitoring of lupus

Factors affecting the test: We use two assays to detect DNA antibodies:

  • Crithidia is a source of pure double stranded DNA and gives a qualitative result
  • The multiplex assay can be contaminated by single stranded DNA, but gives quantitative results

The following configurations can be seen:

  • Crithidia Positive + multiplex flow immunoassay positive = indicates active lupus
  • Crithidia Negative + multiplex flow immunoassay positive = indicates single stranded DNA antibodies, usually triggered by infection or drugs


ICE reference: DS-DNA Antibodies


(Last updated August 28th 2018)