We use cookies to help us improve the website and your experience using it. You may delete and block all cookies from this site at any time. However, please note this may result in parts of the site no longer working correctly. If you continue without changing your settings we will assume you are happy to receive all cookies on this site.


Double stranded DNA antibodies (IgG)

General information

The presence of autoantibodies to double stranded DNA is strongly suggestive of SLE, although they are detected in only 40-60% of patients with this disease.

Specimen transport: At room temperature

Repeat frequency: We recommend not repeating this test more than once a month, unless the patient is
undergoing plasmapheresis

Special precautions: None

Laboratory information

Normal reference range: <13.9 iu/mL

Volume and sample type: 7ml clotted blood

Method: Multiplex flow immunoassay and Immunofluorescence

Turnaround time (calendar days from sample receipt to authorised result): Median - 1, 95th percentile - 5

Clinical information

Indications for the test: Diagnosis and monitoring of lupus

Factors affecting the test: We use two assays to detect DNA antibodies:

  • Crithidia is a source of pure double stranded DNA and gives a qualitative result
  • The multiplex assay can be contaminated by single stranded DNA, but gives quantitative results

The following configurations can be seen:

  • Crithidia Positive + multiplex flow immunoassay positive = indicates active lupus
  • Crithidia Negative + multiplex flow immunoassay positive = indicates single stranded DNA antibodies, usually triggered by infection or drugs


Clinical workstation reference: DNA


(Last updated October 7th 2014)