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Immunodeficiency (T&B Lymphocyte Subsets)

Contact on 0161 276 6440 to discuss extended panels for investigation of Primary Immunodeficiency

General information

These tests are most frequently done in patients who are suspected of having an underlying immunodeficiency or are receiving immunosuppressive drugs. For example, cyclosporine reduces T cell numbers, whilst Rituximab reduces B cell numbers.

The relevant tests required will be specified for each individual case.

Specimen transport: Samples should be received by the lab within 24 hours of venepuncture. Maintain samples at room temp.

Repeat frequency: At significant change of clinical symptoms

Special precautions: None.

Laboratory information

Normal reference range: (95% confidence interval)

CD marker Age Minimum %

Maximum %

Minimum cells/mm3 Maximum cells/mm3
CD3+ Neonates 28 76 600 5000
" 1wk - 2mo 60 85 2300 7000
" 2-5 mo 48 75 2300 6500
" 5-9 mo 50 77 2400 6900
" 9-15 mo 54 76 1600 6700
" 15-24 mo 39 73 1400 8000
" 2-5 y 43 76 900 4500
" 5-10 y 55 78 700 4200
" 10-16 y 52 78 800 3500
" Adults 58 86 700 2100
CD3+CD4+ Neonates 17 52 400 3500
" 1wk - 2mo 41 68 1700 5300
" 2-5 mo 33 58 1500 5000
" 5-9 mo 33 58 1400 5100
" 9-15 mo 31 54 100 4600
" 15-24 mo 25 50 900 5500
" 2-5 y 23 48 500 2400
" 5-10 y 27 53 300 2000
" 10-16 y 25 48 400 2100
" Adults 31 59 300 1400
CD3+CD8+ Neonates 10 41 200 1900
" 1wk - 2mo 9 23 400 1700
" 2-5 mo 11 25 500 1600
" 5-9 mo 13 26 600 2200
" 9-15 mo 12 28 400 2100
" 15-24 mo 11 32 400 2300
" 2-5 y 14 33 300 1600
" 5-10 y 19 34 300 1800
" 10-16 y 9 35 200 1200
" Adults 12 36 200 900
T4:T8 Ratio Adults 0.92 3.72 Ignore Ignore
CD19+ Neonates 5 22 40 1100
" 1wk - 2mo 4 26 600 1900
" 2-5 mo 14 39 600 3000
" 5-9 mo 13 35 700 2500
" 9-15 mo 15 39 600 2700
" 15-24 mo 17 41 600 3100
" 2-5 y 14 44 200 2100
" 5-10 y 10 31 200 1600
" 10-16 y 8 24 200 600
" Adults 3 25 100 500
CD3-CD56+ Neonates 6 58 100 1900
" 1wk - 2mo 3 23 200 1400
" 2-5 mo 2 14 100 1300
" 5-9 mo 2 13 100 1000
" 9-15 mo 3 17 200 1200
" 15-24 mo 3 16 100 1400
" 2-5 y 4 23 100 1000
" 5-10 y 4 26 90 900
" 10-16 y 6 27 70 1200
" Adults 1 19 90 600

 

Paediatric reference ranges are from Comans-Bitter WM, et al, "Immunophenotyping of Blood Lymphocytes in Childhood," J Paediatrics, 1997, 130:388-393

Volume and sample type: EDTA (7mls) blood

Method: Flow Cytometry

Turnaround time (calendar days from sample receipt to authorised result): Median - 2, 95th percentile - 7

Clinical information

Indications for the test: Suspected cellular immunodeficiency

Factors affecting the test: Age (see normal ranges in tables). Acute infection and immunosuppressive drugs will alter T and B lymphocyte numbers.

 

Clinical workstation reference: T AND B CELLS

 

(Last updated December 1st 2014)