Bordetella pertussis PCR (Molecular Microbiology)
General information
Specimen type and collection container:
- Nose and/or throat swab (virus transport medium)
- BAL/Sputum (sterile container)
- NPA (Sterile container)
Specimen transport: Ambient or refrigerated
Minimum volume of sample: Minimum volume
500µL
Special precautions: None known
Laboratory information
Measurement units: Threshold
cycle (CT)
Turn round time to final result (working days):
3-4 days
Clinical information
Clinical decision points: Not
applicable
Factors known to significantly affect the
results: All samples are suitable for overnight
refrigeration only, they must not be stored over a weekend
False negative results may occur for a variety of reasons, for
example inappropriate timing of sample collection, inappropriate
sample, presence of virus below the detectable limit of the assay.
New and emerging variants may also occur which may not be detected
by this assay. Towards the limit of detection of an assay sampling
variation will result in lower reproducibility.
(Last reviewed October 24th 2017)