Candida PCR (Molecular Microbiology)
Collection container (including preservatives):
EDTA blood collection tube
Specimen type: EDTA Blood, Sputum, CSF, BAL,
Specimen transport: Ambient or refrigerated.
Transport at ambient temperature via porter, courier, Royal Mail or
DX compliant with IATA packing instruction 650
Type and minimum volume of sample: 5mL of EDTA
A minimum of 1mL of a bronchoalveolar lavage in a sterile
screw-capped plastic container should arrive at the laboratory
within 1 working day. The sample should not be frozen, but should
be stored at 4°C before dispatch, and kept cool during transport to
the laboratory. Non invasive samples such as sputum and EDTA-blood
may be used if BAL is unobtainable.
A minimum of 0.5mL of whole CSF. Do not centrifuge. Use a small
capacity screw capped container.
Special precautions: Samples should be stored
at 4°C and dispatched as soon as possible after being drawn. If
longer storage is unavoidable, serum or plasma may be stored
frozen, but should not be repeatedly frozen and thawed. In special
circumstances, 0.5mL of serum or plasma can be tested, but for such
small volumes avoid using a large container; use a small capacity
container with a screw cap, such as an Eppendorff tube.
Measurement units: Threshold Cycle (CT)
Biological reference units: Not applicable
Turn round time to final result (working days):
5-7 days. If urgent please contact the laboratory
Clinical decision points: Not
Factors known to significantly affect the
results: All samples are suitable for overnight
refrigeration only, they must not be stored over a weekend.
False negative results may occur for a variety of reasons, for
example inappropriate timing of sample collection, inappropriate
sample, presence of virus below the detectable limit of the assay.
New and emerging variants may also occur which may not be detected
by this assay. Towards the limit of detection of an assay sampling
variation will result in lower reproducibility.