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Chlamydia trachomatis, Neisseria gonorrhoea, Trichomonas vaginalis NAATs (Molecular Microbiology)

General information

Collection container (including preservatives):

cobas® PCR media tube

cobas® PCR Dual Swab Collection Kit

Specimen type: Swab, urine

Collection: Specimens should be collected and handled following the recommended guidelines on the collection packs:

Male and female urine specimens: Male and female urines must be collected in a sterile container and transferred to the cobas® PCR media tube within 24 hours of collection. After transfer, specimens can then be stored at 2-30°C for up to 3 months prior to testing. Urine specimens must fill the cobas® PCR media tube between the 2 black urine fill lines (shown below). If the amount of urine is above or below these lines the specimen will not be tested by the laboratory.

Urine specimen with 2 black urine fill lines

Urine specimen with 2 black urine fill lines

 

Vaginal, throat and anorectal specimens: Only the larger woven polyester swab (shown below) included in the cobas® PCR Dual Swab Collection Kit should be used to collect vaginal, rectal and throat specimens. Specimens may be stored in cobas® PCR media at 2-30⁰C for up to 3 months.

Woven polyester swab used to collect vaginal, throat and anorectal specimens

Woven polyester swab used to collect vaginal, throat and anorectal specimens

 

Endocervical specimens: The larger woven polyester swab (shown above) included in the cobas® PCR Dual Swab Collection Kit should be used first to remove any cervical secretions followed by the smaller flocked swab (shown below) to collect the endocervical specimen.

Flocked swab used to collect endocervical specimens

Flocked swab used to collect endocervical specimens

 

Specimens may be stored in cobas® PCR media at 2-30⁰C for up to 3 months.

Specimen transport: Transport at ambient temperature via porter, courier, Royal Mail or DX compliant with IATA packing instruction 650

Type and volume of sample: 2mL Urine

Special precautions: Patient consent must be obtained for both Chlamdyia and Gonococcal testing.  Single tests cannot be accepted for either Chlamydia or Gonorrhoea. If consent for both cannot be obtained, please contact the laboratory for information on alternative laboratories that provide a single analyte service.

Specimens received more than 3 months after collection will not be tested by the laboratory.

Only the woven polyester swab or flocked swabs contained in the cobas® PCR Dual Swab Collection Kit will be accepted for testing.

Swab specimens received with no swab or 2 swabs will not be tested by the laboratory.cobas® PCR media that has expired will not be tested.

Please ensure the request form clearly states the specimen type and the required test.Please ensure the swabs are not inverted in the tube, i.e. the specimen collection end should be placed in the liquid media.

Laboratory information

Measurement units: Relative Light Units (RLU)

Biological reference units: Not applicable

Turn round time for provisional result (working days): 3 days

Turn round time to final result (working days): 4 days. All reactive tests are confirmed. For urgent tests please contact the laboratory

Clinical information

Clinical decision points: Not applicable

Factors known to significantly affect the results: Samples must be kept at ambient temperature

False negative results may occur for a variety of reasons, for example inappropriate timing of sample collection, inappropriate sample, presence of virus below the detectable limit of the assay. New and emerging variants may also occur which may not be detected by this assay. Towards the limit of detection of an assay sampling variation will result in lower reproducibility.

 

(Last reviewed October 24th 2017)