CMV (Cytomegalovirus) antiviral Resistance markers
CMV viral load positive bloods. Complete CMV antiviral
resistance genotypic screening including
- UL97: Nucleotide sequencing of the CMV phosphotransferase gene
for the identification of mutations encoding resistance to
- UL54: Nucleotide sequencing of the DNA polymerase gene for the
identification of mutations encoding resistance to ganciclovir,
foscarnet and cidofovir
Collection container (including preservatives):
CE marked leak proof container. EDTA blood tube.
Specimen type: EDTA blood
Specimen transport: Ambient or refrigerated
For PCR for confirmation of active CMV infection and monitoring
of antiviral therapy please send 4mL of EDTA blood. This
should be stored at 4°C and dispatched as soon as possible after
being drawn. If longer storage is unavoidable, serum or plasma may
be stored frozen, but should not be repeatedly frozen and thawed.
In special circumstances, 0.5mL of serum or plasma can be tested,
but for such small volumes avoid using a large container but use a
small capacity container with a screw-cap, such as an Eppendorff
Minimum volume of sample: 3.0 mL
Special precautions: If processing is delayed,
refrigeration is preferable to storage at ambient temperature.
Delays of over 48hr are undesirable.
Measurement units: mL Wild type/resistant
Biological reference units: Not applicable
Turn round time for provisional result (working
days): 5 days
Turn round time to final result (working days):
Clinical decision points: Not applicable
Factors known to significantly affect the
results: False negative results may occur for a variety of
reasons, for example inappropriate timing of sample collection,
inappropriate sample, presence of virus below the detectable limit
of the assay. New and emerging variants may also occur which may
not be detected by this assay. Towards the limit of detection of an
assay sampling variation will result in lower reproducibility.