Neisseria meningitidis Minimum inhibitory concentration
Collection container (including preservatives):
Specimen type: Referral of Viable pure cultures
on slopes and transport swabs
Collection including preservative: Not
Specimen transport: Only submit viable isolate
samples in approved packaging (UN3373) which are suitable for Royal
Mail post (airfreight) or commercial couriers such as HAYS DX.
Agar slopes: where possible pure, viable cultures; inoculated on
chocolate (heated) blood agar, blood agar or Dorset egg slopes
after establishing growth by overnight incubation at 37°C.
On occasion it may be necessary to submit an unincubated
culture. This can save time but requires a heavy inoculum to ensure
survival in transport. Please indicate on the request form if the
material (slope) has not been incubated.
Short-term storage of sloped cultures is optimal at 30°C if
there are delays before submission.
Minimum volume of sample: Not applicable
Special precautions: The MICs routinely
determined on submitted isolates are: penicillin, cefotaxime,
rifampicin, ciprofloxacin and sulphonamide (sulphamethoxazole)
using Etest (Biomerieux) gradient diffusion methodology.
Other antibiotic susceptibility tests may be performed on
Measurement units: mg/L
Biological reference units: Not applicable
Turnaround time: 48 hrs if requested
Routine final report is issued within 1-2 weeks
Clinical decision points: Not applicable
Factors known to significantly affect the
results: None known