Neisseria meningitidis: Serogrouping and outer membrane typing
Collection container (including preservatives):
Specimen type: Referral of viable pure cultures
on slopes and transport swabs
Collection including preservative: Not
Specimen transport: Only submit viable isolate
samples in approved packaging (UN3373) which are suitable for Royal
Mail post (airfreight) or commercial couriers such as HAYS DX.
Agar slopes: where possible pure, viable cultures; inoculated on
chocolate (heated) blood agar, blood agar or Dorset egg slopes
after establishing growth by overnight incubation at 37°C.
On occasion it may be necessary to submit an unincubated
culture. This can save time but requires a heavy inoculum to ensure
survival in transport. Please indicate on the request form if the
material (slope) has not been incubated.
Short-term storage of sloped cultures is optimal at 30°C if
there are delays before submission.
Minimum volume of sample: Not applicable
Special precautions: Non-viable cultures:
cultures which are no longer viable may still be considered for
characterisation by molecular based methods after consultation with
the MRU. A heavy inoculum of the inert material on a slope may be
submitted with an appropriate request form.
Measurement units: Not applicable
Biological reference units: Not applicable
Turnaround time: Serogroup telephoned to
sending laboratory within 24 - 72hrs.
The final printed report is submitted within 1 - 2 weeks
Clinical decision points: Not applicable
Factors known to significantly affect the
results: Contaminated culture will delay the