Peritoneal Fluids (Bacteriology)
Continuous ambulatory peritoneal dialysis (CAPD) is used as an
alternative to haemodialysis for the management of patients with
end-stage renal failure.
Collection container (including
preservatives): Withdraw fluid aseptically from
the injection port of the plastic dialysate bag with a sterile
needle and syringe and transfer to a CE marked leak proof
containers and place in sealed plastic bag.
Specimen type: In a sealed plastic bag with a
Cell count = Sterile leakproof container in a sealed plastic bag
containing 20mL of fluid for microscopy.
Culture = Inoculated BacT/ALERT FA bottle (green top).
Collection: Use aseptic technique.
Collect specimens in appropriate CE marked leak proof containers
and transport specimens in sealed plastic bags.
Large volumes or whole dialysate bags may require special
transportation according to local protocols. They should be
transported in rigid, leakproof outer containers.
Specimen transport: Specimens should be
transported and processed as soon as possible
Minimum volume of sample: Minimum volume of
If blood culture bottles are used they should be inoculated
aseptically with 5-10 mL of dialysate
Special precautions: Collect specimens before
antimicrobial therapy where possible.
Measurement units: Cell count
x 106 per litre
Turnaround time: 48 hrs - 7 days for culture.
30 - 60 mins for cell count. Telephone the laboratory in advance
for urgent microscopy
Factors known to significantly affect the
results: If processing is delayed, refrigeration
is preferable to storage at ambient temperature. Delays of over
48hr are undesirable.