Rapid/routine Carbapenemase-Producing Enterobacteriaceae (CPE) Screen (Bacteriology, CMFT)
In response to the increasing numbers of CPE producing clinical
isolates of Enterobacteriaceaethe Infection Control Consultant and
Microbiology department have produced a protocol for CPE screening
and detection. The isolation of a clinical CPE isolate prompts the
Infection Prevention and Control Team to screen all possible
patient contacts to reduce the transmission of resistance enzymes
within the Trust.
Rapid and routine CPE screens are processed on a molecular
platform; culture is only performed on positive samples for
epidemiological and monitoring purposes.
Collection container (including
Swab: Double headed red-topped swab; available from
Microbiology. Please note Charcoal swabs are unsuitable for this
test and will not be processed.
Specimen type: Screening of faeces/rectal
Specimen transport: If processing is delayed,
refrigeration is preferable to storage at ambient temperature.
Delays of over 48hr are undesirable.
Type and minimum volume of sample: Not
Special precautions: None
Measurement units: Threshold Cycle (CT)
Biological reference units: Not applicable
Turnaround time: Rapid CPE Screens : Designated
wards agreed with IPC and Trafford Transfers: 2-4 hours from
receipt into Microbiology Reception. The laboratory MUST be
telephoned prior to the patient(s) being sampled.
Samples should be received in the laboratory before 6pm (Mon-Fri)
and before 4pm (Weekends/Bank Holidays)
Routine CPE Screen: Designated wards agreed with IPC 24-48hrs
Clinical decision points: Not applicable
Factors known to significantly affect the
results: Faecal material must be visible on the cotton tip
of the swab; failure to provide faecal material may produce a false
negative screening result.
Some faecal products may prove inhibitory to the PCR process;
samples will be reported as inhibitory and a repeat will be