Post vasectomy semen checks
There will be an updated information booklet for
patients, available shortly : Vasectomy Semen
Analysis, which can be found in the
related media section of our website.
Post Vasectomy semen analysis (PVSA) is the procedure used to
establish the success of a vasectomy. Contraceptive precautions
should be used until the clinician confirms that the semen sample
is clear of sperm. Assessment of a single sample is acceptable to
confirm vasectomy success if all recommendations and laboratory
methodology are met and no sperm observed.
Special clearance can be given to the patient by the surgeon if
the number of non-motile sperm seen in two assessed samples is
<100 000/ml (a very occasional non-motile sperm seen in a deep
cell chamber). It cannot be provided if any motile sperm are
- The first specimen for examination should be produced at a
minimum interval of 12 weeks post-surgery and after producing at
least 20 ejaculates (British Andrology Society guidelines
- Abstinence should be between 2 and 7 days (3-4 days is
- The sample must be obtained by masturbation (manual
stimulation) and should be collected directly into the specimen
container provided by the laboratory.
- The whole ejaculate must be collected into the
- The complete specimen is needed for this examination, so if any
of the specimen is spilt, the laboratory must be informed and a
repeat specimen may be required.
- A condom and/or artificial lubrication must
not be used for semen collection, as it will kill
- A fresh sample is essential for this
test and must be brought directly to the laboratory within
four hours of it being passed at home.
- The specimen container and request form must be labelled
with the patient's full name, date of birth and the date
and time the specimen was passed.
- If non-motile sperm are observed, further samples must be
examined within 1 hour of production.
- Subsequent specimens produced at the referring doctor's request
should be brought directly to Andrology by the
patient within 1 hour of being